职称：教授
工作地点：生化与微生物教研室
基本信息
暨南大学，生命科学技术学院，教授、博导；
药学院院党委书记、副院长
教育经历
1991/09 - 1994/07，华南理工大学，发酵工程，博士
1988/09 - 1991/07，华南理工大学，发酵工程，硕士
1984/09 - 1988/07，华南理工大学，生物化工，学士
科研与学术工作经历
1994/07 - 至今，暨南大学，生命科学技术学院，历任讲师、副教授、教授
1995/10 - 1996/10，日本大阪大学，生物工学国际交流中心，访问学者
2003/08 - 2005/08，美国加州大学伯克利分校，公共卫生学院，访问学者
主持或参加科研项目（课题）及人才计划项目情况（按时间倒序排序）
1、国家自然科学基金面上项目，人巨细胞病毒编码蛋白UL23调控 HCMV-specific T 细胞增殖、活性及分化的机理，2021.1-2024.12
2、 国家自然科学基金面上项目， 人巨细胞病毒编码蛋白pUL23抑制γ干扰素抗病毒效应的作用机制，2018.1-2021.12。
3、 国家“十三五”重大专项，珠三角地区儿童传染病重要症候群病原谱及危重症病原特征研究，2017.1-2021.12。
4、广东省自然科学基金重点项目，SUMO化修饰介导的人巨细胞病毒与γ干扰素途径相互作用的分子机制，2016.6-2019.6。
5、国家“十二五”重大专项， 长效胰高血糖素样肽-1（GLP-1）在灵长目动物中药代动力学性质及安全性评价，2013/01-2015/12。
6、广东省重大专项，2型糖尿病治疗一类新药长效胰高血糖素样肽-1临床前研究，2012/01-2014/12。
7、国家自然科学基金面上项目， 维生素B12介导生物纳米载体口服传递肠促胰岛素多肽药物，2011/01-2013/12。
8、国家“十一五”重大专项， 依赖血浆凝血酶的肽缓释新策略设计优化长效2型糖尿病多肽药物，2009/01-2010/12。
9、科技部973计划项目， 病毒潜伏感染的分子机制，2011/01-2015/12 （参与）。
10、国家自然科学基金面上项目， 人巨细胞病毒蛋白pUL23抑制病毒自身繁殖的分子机理， 2008/01-2010/12。
代表性研究成果
1. Linyuan Feng, Wanwei Li, Xingyuan Wu, Xiaotian Li, Xiaoping Yang, Yanhong Ran1, Jianguo Wu*, and Hongjian Li*. Human cytomegalovirus UL23 attenuates STAT1 phosphorylation and type I interferon response. Frontiers in Microbiology. 2021 May(accepted)
2. Wenjing Li, Huizi Chai, Jinfeng Deng, Linyuan Feng, Xiaoping Yang, Yanhong Ran, Hongjian Li*.Generation and application of mouse monoclonal antibody against human cytomegalovirus UL23. Viral Immunology. 2020 June. DOI: 10.1089
3. Huizi Chai, Songbin Wu, Jinfeng Deng, Linyuan Feng, Xiaoping Yang, Yanhong Ran, Hongjian Li*. Preparation and Identification of Polyclonal Antibody Against Human Cytomegalovirus Encoding Protein UL23. Protein Expr Purif. 2019 Sep;161, 78-83
4. Feng L, Sheng J, Vu GP, Liu Y, Foo C, Wu S, Trang P, Paliza-Carre M, Ran Y, Yang X, Sun X, Deng Z, Zhou T, Lu S, Li H*, Liu F*. Human cytomegalovirus UL23 inhibits transcription of interferon-γ stimulated genes and blocks antiviral interferon-γ responses by interacting with human N-myc interactor protein.PLoS Pathog. 2018 Jan 29;14(1):e1006867. doi: 10.1371 2018 Jan
5. Liu MY, Liu J, Lai W, Luo J, Liu Y, Vu GP, Yang Z, Trang P, Li H*, Wu J*. Characterization of enterovirus 71 infection and associated outbreak of Hand, Foot, and Mouth Disease in Shawo of China in 2012. Sci Rep. 2016 Dec 12;6:38451. doi: 10.1038/srep38451
6. Liu H, Wang X, Liu S, Li H, Yuan X, Feng B, Bai H, Zhao B, Chu Y, Li H*. Effects and mechanism of miR-23b on glucose-mediated epithelial-to-mesenchymal transition in diabetic nephropathy.Int J Biochem Cell Biol. 2016 Jan;70:149-60
7. Li H*, Ma Y, Chen Y, Sang Y, Zhou T, Qiu M, Huang X, Zhou C, Su Z.A protease-based strategy for the controlled release of therapeutic peptides.Angew Chem Int Ed Engl. 2010 Jul 5;49(29):4930-3
8. Li H , Zhou CX, Su Z . Protease-resistant glucagon like peptide-1 analogs with long-term anti-diabetes type 2 activity.Adv Exp Med Biol. 2009;611:475-6.
9. Li H Li Y, Ni F, Su Z Inhibition of pathogenically-related morphologic transition in Candida albicans by disrupting Cdc42 binding to its effectors.Adv Exp Med Biol. 2009;611:471-2.
10. Li H, Zhou CX, Su JZ Chemical ligation and cleavage on solid support facilitate recombinant peptide purification.Protein Expr Purif. 2006 Dec;50(2):238-46. Epub 2006 Sep 12.
11. Li H, Trang P, Kim K, Zhou T, Umamoto S, Liu F.Effective inhibition of human cytomegalovirus gene expression and growth by intracellular expression of external Q1 guide sequence 2006 RNA Vol.12 No.1 63-72
12. Wang K, Li Y, Zhao G, Wu Y, Zhang X, Li H, Zhou T. Inhibition of human cytomegalovirus DNA replication by small interfering RNAs targeted to UL49.ActaBiochim Biophys Sin (Shanghai). 2013 May;45(5):401-7
13. .Bai Y, Gong H, Li H, Vu GP, Lu S, Liu F.Oral delivery of RNase P ribozymes by Salmonella inhibits viral infection in mice.Proc Natl Acad Sci U S A. 2011 Feb 7 22;108(8):3222-7
14. Bai Y, Li H, Vu GP, Gong H, Umamoto S, Zhou T, Lu S, Liu F. Salmonella-mediated delivery of RNase P-based ribozymes for inhibition of viral gene expression and replication in human cells.Proc Natl Acad Sci U S A. 2010 Apr 20;107(16):7269-74
15. Zhang W, Li H, Li Y, Zeng Z, Li S, Zhang X, Zou Y, Zhou T.Effective inhibition of HCMV UL49 gene expression and viral replication by oligonucleotide external guide sequences and RNase P.Virol J. 2010 May 18;7:100
16. Zeng Z, Li H, Li Y, Cui Y, Zhou Q, Zou Y, Yang G, Zhou T.Effective inhibition of human cytomegalovirus gene expression by DNA-based external guide sequences.Acta Biochim Biophys Sin (Shanghai). 2009 May;41(5):389-98
17. Zhou T, Xiao X, Xu B, Li H, Zou Y.Overexpression of SSTR2 inhibited the growth of SSTR2-positive tumors via multiple signaling pathways.Acta Oncol. 2009;48(3):401-10.
18. Bai Y, Trang P, Li H ,Kim K, Zhou T, Liu F. Effective inhibition in animals of viral pathogenesis by a ribozyme derived from RNase P catalytic RNA. Proc Natl Acad Sci U S A. 2008 Aug 5;105(31) :10919-24.
19. Su ZD , Li HJ , Li Y and Ni F, Inhibition of pathogenically-related morphological transition in Candida albicans through intervening interactions of Cdc42 with its effectors: Impact on signal transduction therapy ,Chemistry & Biology (Cell Press), 2007，Nov.14(11):1273-82
20. Guo F, Huang X, Li S, Sun L, Li Y, Li H, Zhou Y, Chu Y, Zhou T.Identification of prosaposin as a novel interaction partner for Rhox5.J Genet Genomics. 2007 May;34(5):392-9
21. Yang YH,Li H, Zhou T, Kim K, Liu F Engineered external guide sequences are highly effective in inducing RNase P for inhibition of gene expression and replication of human cytomegalovirus Nucleic Acids Research,2006, Vol. 34, No.2575-583
22. Zhang WJ, Li HJ , Li YQ, He HK, Tang DS, Zhang X, Zhou TH.Construction of An Effective M1GS Ribozyme Targeting HCMV UL97 mRNA Segment in Vitro.Acta Genetica Sinica, November 2005,32(11),1205-1212
23. Su YZ, Li HJ, Li YQ, Chen HJ, Tang DS, Zhang X, Jiang H, Zhou TH. In Vitro Construction of Effective M1GS Ribozymes Targeting HCMV UL54 RNA Segments. Acta Biochimica et Biophysica Sinica, 2005, 37(3):210-21
会议论文
1. H. LI#*, S. WU, L. FENG, H. CHAI, X. YANG, Z. DENG. IFN-γ-dependent transcriptome altered by human cytomegalovirus-encoding UL23 protein on human foreskin fibroblast cells(HFF) using RNA-seq. ASHG 2019 Annual Meeting in Houston, TX , USA. October 15- 19.
2. Hongjian Li #*, Linyuan Feng , Chingman Foo ,Songbin Wu,Yanhong Ran, Xiaoping Yang, Zemin Deng, Tianhong Zhou. Human cytomegalovirus UL23 protein regulates transcription of interferon-γ stimulated genes. ASHG 2018 Annual Meeting in San Diego CA, USA. October 16- 20.
3. Li, H#*. Li, S. Yang, L. Feng, Y. Ran, X. Yang, T. Zhou, Z. Deng. Transcriptome altered by lytic human cytomegalovirus infection on human foreskin fibroblast cells(HFF) using RNA-seq. ASHG 2017 Annual Meeting in Orlando FL, USA. October 18- 22.
4. Li Hongjian#*, Ran Yanhong，Jin Jian，Yang Xiaoping，Zhou Tianhong，Deng Zemin. Modulation of gut microbiota by GLP-1 receptor agonist in the high-fat diet mice. ASHG 2016 Annual Meeting in Vancouver,Canada. October 18-22.
5. Li Hongjian#*, Wang Bo, Zhou Tianhon g, Deng Zemin. Survey of Human Cytomegalovirus Gene Polymorphism in Infants Infected Congenitally or Postnatally from Gunagzhou, China. ASHG 2015 Annual Meeting in Baltomore MD.USA. October 06- 11.
6. Li Hongjian#*, Yang Dan, Feng Linyuan, Ran Yanhong , Liu Haifeng, Zhou Tianhong, Deng Zemin. Studies on the function of HCMV-encoded essential gene UL49. ASHG 2014 Annual Meeting in San diego CA .USA. October 18- 22.
7. Li Hongjian#*，Zeng Baojuan, Li Jidong, Liu Haifeng, Zhou Tianhong, Deng Zemin. Association of Human Cytomegalovirus pUL23 Protein with RACK1 Results in Dissociation of STAT-1 from RACK1-STAT-1 Complex. ASHG 2013 Annual Meeting in Boston, MA.USA. October 22-26.
8. Li Hongjian#*, Jiang Xuefang, Sun Liang, Zhou Tianhong, Deng Zemin. Effective inhibition of human cytomegalovirus gene expression and lytic replication by DNA-based external guide sequence. ASHG 2012 Annual Meeting in San Francisco, CA. USA. November 6-10.
授权发明专利
1. 李弘剑、周天鸿、苏正定 “胰高血糖素样多肽-1类似物及其应用”，2011.05.25，中国，ZL200510102363.7
2. 李弘剑、周天鸿、苏正定 “胰高血糖素样多肽-1类似物及其应用” （分案）,2012.02.22，中国，ZL201010291350.X
3. 李弘剑、周天鸿、苏正定 美国专利“METHOD FOR SUSTAINEDLY RELEASING BIOACTIVE PEPTIDES AND APPLICATION THEREOF”。（申请案号12/236,343）
4. 李弘剑、周天鸿、苏正定 “一种生物多肽药物的缓释方法”,2013.07.31，中国，ZL 200810028614.5
5. 李弘剑、蒋德旗、冉艳红、苏正定、周天鸿 “利用乳糖诱导pMFH载体生产重组蛋白的发酵方法”,2012.11.21，中国，ZL201010221511.8
6. 李弘剑、郑飞、冉艳红、苏正定、周天鸿 “大规模纯化含有His Taq和甲酸水解位点的融合蛋白的方法”，2014.10.08，中国,ZL201110032128.2
7. 李弘剑、王从峰、冉艳红、苏正定、周天鸿 授权专利“含有胰高血糖素样肽-1的融合蛋白及制备方法与应用”，2013.11.06，中国，ZL 201210007118.8
8. 李弘剑、冉艳红、刘天祥、陈美云、李万维、杨小苹“载有药物的维生素B12衍生物自组装纳米微粒及制备方法与应用”，2020.11.13，中国，ZL 201610992427.3
9.李弘剑、陈美云、张忠民、冉艳红、刘天祥、李万维、杨小苹“可自主装形成纳米微粒的维生素B12衍生物及制备方法与应用” 2020.11.11，中国，201610993933.4
社会兼职
广东省遗传学会副理事长、中国遗传学会理事
